PENGARUH α- BENZIL AMINO PURINA DAN α- ASAM ASETAT NAFTALENA TERHADAP PEMBENTUKAN TUNAS TANAMAN NILAM (Pogostemon cablin Benth.) SECARA IN-VITRO

Rozaliana Rozaliana, Luthfi A Aziz Mahmud Siregar, Eva Sartini Bayu

Abstract


This research aims to determined the concentration of the most effective growth regulator for bud
induction patchouli plant by in-vitro methode. The research was done in the laboratory of plant
tissue culture, agriculture faculty university of north sumatra, medan from January 2012 to june
2012 using factorial completely randomized design with 2wo factors that is giving BAP (0, 0.5, 1
and 1.5 mg/l) and NAA (0, 0.2, 0.4 and 0.6 mg/l). The measured parameters were percentage of
eksplan living, the percentage of contamination, the percentage of eksplan forming buds, bud
height, roots number, root length, leaves number, noods number, time of initiation, appearance of
callus, colour of callus and texture of callus. The result showed that giving BAP and NAA
significantly affected on the buds initiation time parameters with the best time of initiation is 7 days
((0.6 mg/l NAA and 1.5mg/l BAP and the highest number of root that 8 pieces (0.2 NAA mg/l and
BAP 1.5mg/l )and did not significanly on others parameter. The best medium for bud induction
was not found yet.
Key words : NAA, BAP, patchouli

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Program studi Agroteknologi

Fakultas Pertanian

Jl. Prof. A. Sofyan no. 3

Kampus Universitas Sumatera Utara

Padang Bulan- 20155

Medan-Sumatera Utara

E ISSN (2337-6597)