UJI ANTAGONISME ISOLAT MUTAN Sclerotium rolfsii SACC. TERHADAP ISOLAT TIPE LIAR Sclerotium rolfsii SACC. DI LABORATORIUM

Nurainun Nasution, Hasanuddi Hasanuddi, Darma Bakti Darma Bakti

Abstract


Antagonism test mutated isolate of Sclerotium rolfsii Sacc. against wild type isolate of
Sclerotium rolfsii Sacc. in laboratory. This research aims to determine ability from mutated isolate
of S. rolfsii to inhibit wild type isolate of S. rolfsii’s growth in laboratory. It was conducted in Plant
Pathology Laboratory, Faculty of Agriculture, University of Sumatra Utara, Medan from September
until November 2012. It was done by using Completely Randomized Design Non Factorial with 7
treatments and 3 replications. This research’s result showed that highest percentage of inhibiting
zones contained at 20 and 25 minutes UV irradiated isolate (M4 and M5) at 63.66 % and 60.95 %
and the lowest were at 30 and 15 minutes UV irradiated isolate (M6 and M3) at 52.80 % and
55.17 %. Macroschopic of S. rolfsii experience of the change at 15 and 30 minutes UV irradiated
isolate (M3 and M6) were in the form of colony more dense and compact, myselium like cotton and
hyphae in the form of refinement. The highest diametre from mutated isolate of S. rolfsii contained
at 20 minutes UV irradiated isolate (M4) at 6.63 cm and the lowest were at 30 minutes UV
irradiated isolate (M6) at 4.76 cm. The highest wide of growth from mutated isolate of S. rolfsii
contained at 20 minutes UV irradiated isolate (M4) at 34.15 cm2 and the lowest were at 30 minutes
UV irradiated isolate (M6) at 18.54 cm2.
Keywords: S. rolfsii, antagonism, pathogenecity, sclerotia.

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Program studi Agroteknologi

Fakultas Pertanian

Jl. Prof. A. Sofyan no. 3

Kampus Universitas Sumatera Utara

Padang Bulan- 20155

Medan-Sumatera Utara

E ISSN (2337-6597)