Analisis Keragaman Genetik Andaliman (Zanthoxylum acanthopodium DC.)Sumatera Utara Menggunakan Marka RAPD (Random Amplified Polymorphic DNA)

Ayu Oshin Yap Sinaga, Lollie Agustina P. Putri, Luthfi Aziz M. Siregar

Abstract


The aim of the research was to analyze the genetic diversity of the endemic andaliman in
North Sumatera based on RAPD markers. A total of 30 accessions andaliman originated from
the various region in North Sumatera, i.e.: Dairi, Karo Highland, Simalungun. The RAPD
analyse was used with 3 random primer: OPH-06, OPC-12 and OPN-03. These three primers
showed 32 bands, on which 31 were polymorphic (95.23%). Further genetic diversity
coefficient and the filogenetic dendogram were obtained using the Darwin 5.05 software. The
results showed that 30 accessions of andaliman were clustered in three groups. Each group
consisted of the accessions of three regions from different altitudes. There were 30 accessions
of three regions studied showed a high genetic diversity.

Keywords: Andaliman, genetic diversity, random amplified polymorphic DNA

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Program studi Agroteknologi

Fakultas Pertanian

Jl. Prof. A. Sofyan no. 3

Kampus Universitas Sumatera Utara

Padang Bulan- 20155

Medan-Sumatera Utara

E ISSN (2337-6597)